Prestained SDS-PAGE Standard Solution for Molecular Weights 30,000-120,000 

Prestained markers are supplied as a ready-to-load mixture for SDS-PAGE and Western blotting that are visible without any subsequent staining. Prestained markers transferred onto nitrocellulose, nylon or PVDF provide a means to determine the molecular weight of an immunostained protein on a Western blot and provide a visual check of transfer efficiency of protein to the membrane. Prestained markers also provide a visual monitor of protein migration during electrophoresis.

The molecular weights of the proteins are altered by the covalent attachment of dye. The apparent molecular weights of the conjugated proteins are determined and listed on the label. When precise molecular weight determinations are necessary, SigmaMarkers should be used.

Unstained protein molecular weights in Daltons
Triosephosphate isomerase: 26,600
Lactic dehydrogenase: 36,500
Ovalbumin: 45,000
Pyruvate kinase: 58,000
Lactoferrin from Human Milk: 90,000
β-Galactosidase: 116,000

aqueous glycerol solution

1 vial sufficient for 100 uses (Western Blotting)

1 vial sufficient for 25 uses (Large format gels)

1 vial sufficient for 50 uses (Mini format gels 10 × 10 cm)

dry ice

−70°C

B

3