Puromycin dihydrochloride from Streptomyces alboniger 

Recommended for use as a selection agent at a range of 1-10 μg/ml.

Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.

Nucleoside antibiotic. Protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3'-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells. Acts very quickly and can kill 99% of cells within 2 days, the resistance gene (puromycin acetyltransferase) gives very effective protection.

≥98% (TLC)

powder

H₂O: 50 mg/mL

cell culture tested

−20°C

de la Luna, S., Ortin, J., pac gene as efficient dominant marker and reporter gene in mammalian cells. Meth. Enzymol. 216, 376, (1992)

Nathans, D., Gottlieb, D., Shaw, P.D. Antibiotics New York, NY , (1967) 1, 259

Beil. 26,IV,3704

3

AU7355000