Protease Inhibitor Cocktail 

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, and aminopeptidases, and thermolysin-like activities. Formulated with no metal chelators that might inhibit binding of histidine-tagged proteins to metal affinity resins (IMAC). Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, pepstatin A, E-64, and phosphoramidon.

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

DMSO solution

−20°C

1

185 °F

85 °C