Monoclonal Anti-HLA-DR−PE antibody produced in mouse 

HLA-DR is located on chromosome 6. It is composed of a heavy and light chain, which are two non-covalently associated subunits of molecular weight 33-36 kDa and 24-30 kDa, respectively. These class II antigens are expressed primarily on B cells, and on monocytes and activated T cells, and function in cell-cell recognition in regulation of the immune response.

GM-1500 human tumor cell line

Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.

Recognizes HLA-DR, a product of the human major histocompatibility complex. The antibody is specific for a non-polymorphic HLA-DR determinant.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

purified immunoglobulin

HK14

buffered aqueous solution

human

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG2a

human ... HLA-DRA(3122), HLA-DRB1(3123)

wet ice

2-8°C

Shipp, M.A., et al. J. Immunol. 131, 2458, (1983)

Shipp, M.A., et al. Human Immunol. 16, 24, (1986)

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