Application
Used to deglycosylate protein.
Biochem/physiol Actions
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Packaging
Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions
Legal Information
N-Glycanase is a registered trademark of Genzyme Corporation, Boston, MA, USA. N-Glycanase is covered by US Patent No 5,238,821.
Unit Definition
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
Legal Information
N-Glycanase is a trademark of Genzyme Corp.
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