Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain 

Protein determined by Warburg-Christian

May be used to assay the protein activator, calmodulin.

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Package size based on activated units

Affinity purified preparation which has been depleted of calmodulin.

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Lyophilized powder containing Tris-HCl buffer salts and lactose

lyophilized powder

1-5 units/mg protein (without added calmodulin)

mol wt ~60 kDa

Protein, ~5% Lowry

2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase <0.1%

5′-ATPase <0.2%

inorganic pyrophosphatase <0.8%

cow ... PDE1A(281969)

−20°C

Houslay, M.D. Semin. Cell. Dev. Biol. 9, 161-167, (1998) abstract

Chock., S.P. and Huang, C.Y., An optimized continous assay for cAMP phosphodiesterase and calmodulin. Anal. Biochem. 138, 34-43, (1984) abstract

Drummond, G.I. And Yamamoto, M., Boyer, P.D., ed. The Enzymes, (1971)

3

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