PeroxiDetect™ Kit 

Kit contains reagents for 100 tests of aqueous peroxide and 100 tests of organic peroxide.

For the determination of aqueous peroxides and lipid hydroperoxides. Peroxides serve as a source for hydroxyl or peroxyl reactive radicals. These radicals can interact with DNA, proteins or lipid components of cells, causing cell damage that could lead to cell death. The measurement of peroxides in biological systems is an important factor in determining the degree of free radicals present in specific tissues. Lipid peroxidation has been proposed to contribute to various pathophysiological cell and tissue abnormalities. For example, increased levels of lipid peroxidation products in red blood cells were found to correlate well with the onset of diabetes mellitus in pregnant women. Cholesterol hydroperoxides accumulate in patients having excessive blood alcohol. Measurement of hydrogen peroxide in tissues has been used to study several aspects of free radical damage such as skin aging as induced by UV light and the effect of H₂O₂ as an inducer of elevated tyrosinase levels in melanoma cells. H₂O₂ has been shown to be a potent mitogen for growth-arrested cultured human aortic smooth muscle cells. The PeroxiDetect kit is capable of detection of H₂O₂ in aqueous solutions in the range of 1-7 nanomoles per reaction volume or of lipid hydroperoxides in organic solvents in the range of 1-16 nanomoles per reaction volume. This kit is based on the fact that peroxides will convert Fe²⁺ ion to Fe³⁺ ion at acidic pH. The Fe³⁺ ion will form a colored adduct with xylenol orange which is observed at 560 nm.

2-8°C

Carone, D., et al., Lipid peroxidation products and antioxidant enzymes in red blood cells during normal and diabetic pregnancy. Eur. J. Obstet. Gyn. Rep. Biol. 51, 103-109, (1993)

Adachi, J., et al., Abnormality of very long-chain fatty acids of erythrocyte membrane in alcoholic patients. Alcohol. Clin. Exp. Res. 22, 103S, (1998) abstract

Masaki, H., Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem. Biophys. Res. Commun. 206, 474, (1995) abstract

Herbert, J.M., The mitogenic effect of H₂O₂ for vascular smooth muscle cells is mediated by an increase of the affinity of basic fibroblast growth factor for its receptor. FEBS Lett. 395, 43, (1996) abstract

Karg, E., et al., Hydrogen peroxide as an inducer of elevated tyrosinase level in melanoma cells. J. Invest. Dermatol. 100, 209S-213S, (1993) abstract

Adachi, J., et al., Cholesterol hydroperoxides in erythrocyte membranes of alcoholic patients. Alcohol. Clin. Exp. Res. 23, 96S-100S, (1999) abstract

UN 3316 5.2/PG 3

Aqueous Peroxide Color Reagent 100 mL

tert-Butyl hydroperoxide 1 mL

Ferrous Ammonium Sulfate Reagent 2 mL

Hydrogen peroxide 1 mL

Organic Peroxide Color Reagent 1 bottle