Protease Fluorescent Detection Kit 

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases)

The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.

Supplied with sufficient reagents for up to 200 one mL assays.

Detection limit: approx. 5 ng trypsin

dry ice

−20°C

UN 3316 9/PG 2

Incubation buffer 25 ml

FITC-casein solution 5 ml

Assay buffer 200 ml

Protease standard (trypsin) 20 μg

FITC control 50 mg

Trichloroacetic acid (TCA) solution 30 ml