PKH2 Green Fluorescent Cell Linker Kit for Phagocytic Cell Labeling 

For additional technical details on PKH Fluorescent Cell Linker Dyes including a comprehensive bibliography, please visit www.sigma-aldrich.com/pkh.

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for more than 21 days in vivo. Intraperitoneal or intravenous injections of the PKH2 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

U.S. Patent Numbers: 4,783,401; 4,762,701; 4,859,584.
Distributed for Phanos Technologies.

UN 3316 9/PG 3

PCLDIL, Diluent B for Phagocytic cell labeling

Diluent B 6×10 mL

PKH2 Cell Linker in ethanol 0.5 mL