GenElute^™ Plasmid Miniprep Kit 

The GenElute Plasmid Purification Kits offer simple, rapid, and cost-effective methods for isolating plasmid DNA from E. coli cultures. These kits combine silica-based membrane technology and the convenience of a spin column format. These kits also recover up to 20 μg of high copy plasmid DNA per mL of overnight culture.

For additional information, please see www.sigma-aldrich.com/genelutehp.

• 40% more preps per kit than the leading supplier
• Purify up to 20 μg of plasmid DNA per mL of culture
• Purified plasmid DNA in less than 30 minutes for up to 24 preps
• Faster than gravity flow anion exchange columns
• No detectable genomic DNA or RNA contamination
• No phenol/chloroform extraction or alcohol precipitation required
• Contains additional wash buffer for use with EndA⁺ E. coli bacterial strains (e.g., HB101, JM101, BL21)

Bacterial cells are harvested via centrifugation, subjected to a modified alkaline-SDS lysis procedure and the DNA adsorbed onto silica in the presence of high salts. Contaminants are then removed by a simple wash step.
Finally, the bound DNA is eluted in water or Tris-EDTA buffer. The recovered plasmid DNA is predominately in its supercoiled form. There is no visual evidence of genomic DNA or RNA contamination. The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

sufficient for 70 purifications

UN 3316 9/PG 3