p38 MAPK ELISA 

The sensitivity is <16 pg/mL of human p38 MAPK, which is 10x more sensitive than immunoblotting. Linear regression yielded a correlation coefficient of 0.99.

p38 MAPK ELISA specifically measures human, monkey, and mouse p38 MAPK.

A solid phase sandwich enzyme-linked immunosorbent assay (ELISA).

p38 MAP kinase (MAPK), also known as a CDC-2-related protein kinase or CSBP (cytokine suppressive anti-inflammatory drug binding protein) regulates many cellular processes including inflammation, cell differentiation, cell growth and death. p38 MAP kinase is the human homolog of the S. cerevisiae gene Hog1, which is a MAPK required for growth under high-osmolarity conditions. The kit is designed to detect and quantify the levels of phosphorylated and non-phosphorylated p38 MAPK from cell lysates. It detects native and recombinant p38 protein.

The assays are a sensitive, quantitative, and economical alternative to traditional immunoblotting or bioassays. They are fast (4 hours), easy to perform (precoated plates, room temperature incubations, no proprietary equipment required) and the results are reproducible from lot-to-lot.

sufficient for 96 multiwell tests

wet ice

2-8°C

Han, J., et al., Molecular cloning of human p38 MAP kinase. Biochim. Biophys. Acta 1265, 224-227 , (2000)

Bulavin, D. V., et al.,, Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase. Nature 411, 102-107, (2001) abstract

Han, J., et al., Molecular cloning of human p38 MAP kinase. Biochim. Biophys. Acta 1265, 224-227, (2000)

UN 3316 9/PG 3

p38 MAPK Standard (lyophilized, human, full length recombinant p38 MAPK protein expressed in E. coli) 2 vl

Standard Diluent Buffer 1x25 mL

Anti-p38 MAPK-Coated 96 Well Plate 1xEA

Anti-p38 MAPK Antibody 1x11 mL

Anti-Rabbit IgG-Horseradish Peroxidase (HRP) Concentrate (100x) 1 Vl

HRP Diluent

Wash Buffer Concentrate 25x 1x100 mL

Stabilized Chromogen, Tetramethylbenzidine (TMB) 1x25 mL

Stop Solution 1x25 mL

Plate Covers, Adhesive Strips 3 EA