PhosphoProfile^™ I Phosphopeptide Enrichment Kit 

The PhosphoProfile I Phosphopeptide Enrichment Kit is designed to increase the concentration of phosphopeptides from tryptic digests by immobilized metal affinity chromatography (IMAC). The enriched solution helps to overcome the limitations of mass spectrometry of phosphopeptides. The provided reagents are compatible with downstream LC-MS and MALDI-TOF MS analysis.

• Low non-specific binding - high data confidence
• No bias in phosphorylation states - accurate representation of phosphorylation types present for sample comparisons
• Fast sample processing - reduces time to data generation from sample collection
• Complete optimized set of reagents and enzymes - Reduce cost and effort with total sample management in one package

The kit is a convenient collection of all materials necessary to perform phosphopeptide enrichments from crude samples following tryptic digestion. The supplied phosphopeptide capture matrix is a novel Ga (III) chelate silica based on Sigma-Aldrich's proprietary nitriloacetic acid (NTA) analog. The matrix is packed into spin columns for easy, microscale affinity capture of phosphopeptides.

PhosphoProfile is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

sufficient for 24 purifications

wet ice

2-8°C

F,C

11-20/21/22-34-42

16-26-36/37/39-45

UN 3316 9/PG 3

PHOS-Select^™ Gallium Spin Column

Bind/Wash Solution for PhosphoProfile^™ I

Elution Solution for PhosphoProfile^™ I

Monophosphopeptide bovine

Tetraphosphopeptide from bovine milk

Trypsin from porcine pancreas, Proteomics Grade, Dimethylated T6567

Trypsin Solubilization Reagent

Enzyme Reaction Buffer

Collection Tubes