Protein Tyrosine Kinase (PTK) Assay Kit, Non-Radioactive 

Enables the detection, measurement and characterization of protein tyrosine kinase (PTK) activity during purification (column fractions, etc.) of natural and recombinant PTKs, in cell and tissue extracts and immunoprecipitates. This kit is based on an ELISA assay format using the universal PTK substrate poly-Glu-Tyr (PGT). Detection is achieved with a phosphotyrosine specific monoclonal antibody (clone PT-66) conjugated to peroxidase (HRP). EGF Receptor (EGFR) is also included as a positive tyrosine kinase control.

sufficient for 96 reactions

dry ice

−20°C

UN 3316 6.1/PG 3

Adenosine-5'-Triphosphate (ATP 1×5 mg)

Epidermal Growth Factor Receptor (EGFR) 1×100 units

Monoclonal Anti-Phosphotyrosine-Peroxidase Conjugate 1 vial

Protein Tyrosine Kinase (PTK) Substrate, polyGlu-Tyr (PGT) 1×5 mg

SIGMA FAST™ OPD 5×5 sets

Tyrosine Kinase Buffer (10x) 1×15 mL

Washing Buffer (PBS-Tween 20) 1 package

96-well Microtiter Plate, with a strips holder and plate cover 1 each