Biochem/physiol Actions
For Quantitative Flow Cytometry. The QC Windows is a unique microbead standard used to achieve a unified setup protocol for all cytometers. This practice effectively normalizes all fluorescence intensity data across laboratories and different models of cytometers. The QC Windows Kit contains a certified blank microbead and a microbead standard for flow cytometry. The two reagents are uniform in light scatter characteristics and are provided in separate vials. The certified blank microbead is used to determine the fluorescent threshold of the instrument (noise level). The microbead standard is labeled with both FITC and PE and has lot specific target channel values assigned. These two reagents are used to determine the appropriate PMT voltage setting for any cytometer. The QC Windows Kit establishes a common Window of Analysis to be employed across all laboratories and instruments, therefore, permitting the direct comparison of histogram data.
Legal Information
QC Windows is a trademark of Flow Cytometry Standards Corp.