SYBR^® Green Quantitative RT-PCR Kit 

The SYBR Green Quantitative RT-PCR Kit delivers high specificity and reproducibility. SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma's SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq. The kit has been optimized for use with both plate/tube real-time instruments and with the Roche LightCycler capillary instrument. A reference dye is provided in a separate vial to be used in ABI Detection Systems.

The SYBR Green Quantitative RT-PCR kit provides a highly sensitive method for the quantitative analysis of gene expression. Sigma's QRT-PCR ReadyMix^™ combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart^™ Taq in a convenient ReadyMix. The kit includes M-MLV RT, an extremely robust enzyme which has the ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C), SYBR Green I dye, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer and stabilizers and is provided as a 2× concentrate.

JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR^® Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR^® is a registered trademark of Molecular Probes, Inc.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

SYBR is a registered trademark of Molecular Probes, Inc.

1 kit sufficient for 100 50-μl PCR reactions

wet ice

−20°C

Z702439, A-Z of Quantitative PCR

SYBR^® Green Taq ReadyMix™ for Quantitative RT-PCR

Molony Murine Leukemia Virus Reverse Transcriptase (M-MLV™)

10X PCR Buffer P2192

25 mM MgCl₂ M8787

Reference Dye for Quantitative PCR, 100 ×, solution R4526