Quantitative RT-PCR ReadyMix^™ 

The Quantitative RT-PCR ReadyMix kit includes Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), an extremely robust enzyme which has the ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C). In addition, the ReadyMix is blended with JumpStart Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers, and is provided as a 2× concentrate for convenience. The kit provides high specificity, reduced risk of contamination, and increased reproducibility.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

1 kit sufficient for 100 50-μl PCR reactions

wet ice

−20°C

Xi,B

36/37/38

26-36

Z702439, A-Z of Quantitative PCR

Probe Based qRT-PCR ReadyMix

Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT)

10X PCR Buffer P2192

Magnesium chloride solution, PCR Reagent M8787

Reference Dye for Quantitative PCR, 100 ×, solution R4526