BamH  I from Bacillus amyloliquefaciens H 

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).

Recognition sequence: 5′-G/GATCC-3′
Ligation and recutting results: After 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and 95%recut.
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.

Solution in 10 mM Tris-HCl, pH 7.4 , 1 mM EDTA, 1mM dithioerythritol, 300 mM KCl, 0.01% Polydocanol (v/v), 50% glycerol (v/v), at 4°C

buffered aqueous glycerol solution

10,000 units/mL

wet ice

−20°C

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Wilson, G.A., et al., Isolation of a sequence-specific endonulease (Baml) from Bacillus amyloliquefaciens.. J. Mol. Biol. 97, 123-125, (1975) abstract

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LIG2, QuickLink^™ DNA Ligation Kit

D2886, DNA Ligase from T4-infected Escherichia coli

B8781, Restriction Endonuclease Buffer SB