RIPA Buffer 

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RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins' immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview^™ Affinity Gels.

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL^® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.

EZview is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

IGEPAL is a registered trademark of General Dyestuff Corp.

protease contamination, tested

2-8°C

Ed Harlow and David Lane Antibodies: A Laboratory Manual, (1988), 449

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