R0754
Sigma

Sal I from Streptomyces albus G

buffered aqueous glycerol solution

CAS Number

81295-38-7

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00165804

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Specificity

Recognition sequence: 5′-G/TCGAC-3′
Ligation and recutting results: After 2-10-fold Sal I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.

Physical form

Solution in 10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 50% glycerol (v/v), 10 mM dithioerythritol, at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Arrand, J.R., et al., A new restriction endonuclease from Streptomyces albus G. J. Mol. Biol. 118, 127-135, (1978) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany