R1258
Sigma

Kpn I from Klebsiella pneumoniae

buffered aqueous glycerol solution

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CAS Number

81295-27-4

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166575

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SL (B3782).

Comment: Kpn I shows abnormal DNA cleaving patterns (star activity) in reactions containing high (>5%) glycerol, >5% (v/v); excess enzyme and high pH (>8.0).
The enzyme will not cleave DNA containing 3′-residue of 5-methylcytosine.

Specificity

Recognition sequence: 5′-GGTAC/C-3′
Ligation and recutting results: After 2-10-fold Kpn I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Tomassini, J., et al., Recognition sequence of restriction endonuclease Kpnl from Klebsiella pneumoniae. Nucleic Acids Res. 5, 4055-4064, (1978) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany