Monoclonal Anti-Ryanodine Receptor antibody produced in mouse 

partially purified chicken pectoral muscle ryanodine receptors (RyR)

The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.

Diluted ascites fluid containing 0.05% sodium azide

ascites fluid

34C

antigen mol wt 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)

chicken, human, bovine, sheep, canine, rat, mouse, rabbit, frog, fish

immunoblotting: 1:5,000

immunohistochemistry (frozen sections): 1:1,000

IgG1

human ... RYR1(6261), RYR2(6262)

mouse ... Ryr1(20190), Ryr2(20191)

rat ... Ryr1(114207), Ryr2(84025)

dry ice

−20°C

Airey, J.A, Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle. J. Biol. Chem. 265, 14187-14194, (1990) abstract

-