R1636
Sigma

EcoR II from Escherichia coli MV1 193

buffered aqueous glycerol solution

CAS Number

81295-15-0

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00163534

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Linkage

Isoschizomers : BstN I, Apy I, BsiL I, BstO I

Other Notes

Comment: Ad-2 DNA is the substrate in the assays. EcoR II will only partially cleave DNA isolated from E. coli strains that have the dcm methylase (dcm ⁺ strains).

Specificity

Recognition sequence:5′-/CC(^A_T)GG-3′
Ligation and recutting results: After 2-10-fold EcoR II overdigestion of 1 μg Ad-2 DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: 65 °C for 10 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.0, 1 mM EDTA, 300 mM KCl, 1 mM dithioerythritol, 0.01% polydocanol, 50% glycerol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Boyer, H.W., et al., DNA substrate site for the EcoR II restriction endonuclease and modification methylase. Nature 244, 40-43, (1973)

Bigger, C.H., et al., Recognition sequence of a restriction enzyme. Nature 244, 7-10, (1973)

Safety

WGK Germany