R1761
Sigma

Cfo�I from Clostridium formicoaceticum

buffered aqueous glycerol solution

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CAS Number

81811-52-1

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166595

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SL (B3782).

Linkage

Isoschizomer: Hha I

Specificity

Recognition Sequence: 5′-GCG/C-3′
Ligation and recutting results: After 2-10-fold Cfo�I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Not complete at 65�°C for 10 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.02% polydocanol, 0.1mM PMSF, 50% glycerol (v/v), at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Makula, R.A., et al., A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway. Nucleic Acids Res. 8, 3125-3131, (1980) abstract

Safety

WGK Germany