R2631
Sigma

Pvu II from Proteus vulgaris

buffered aqueous glycerol solution

zoom

1 of 1

CAS Number

81295-34-3

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166580

EG/EC Number

279-736-1

Expand/Collapse All

Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SM (B3158).

Comment: This enzyme exhibits star activity when used under conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH >8.0.
Pvu II does not cut CAGm⁴CTG or CAGm⁵CTG.

Specificity

Recognition sequence: 5′-CAG/CTG-3′<
Ligation and recutting results: After 2-10-fold Pvu II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity not completely destroyed at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.7, 1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% Triton X-100 (v/v) at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Gingeras, T.R., et al., Two new restriction endonucleases from Proteus vulgaris.. Nucleic Acids Res. 9, 4525-4536, (1981) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany

RTECS

RF0758100