RapidTransit^™ Transformation Kit 

• Fast, economical preparation of competent cells
• Overcomes costs and strain limitations of commercially available cells
• Suitable for a variety of E. coli strains
• Eliminates complex buffer preparation
• Efficiencies suitable for cloning

The RapidTransit™ Transformation Kit provides an economical and streamlined method to prepare chemically competent Escherichia coli cells at efficiencies suitable for standard cloning. RapidTransit reagents eliminate complex buffer preparation and lengthy incubation periods, while reducing the number of procedural steps.

This kit has been used successfully to prepare competent cells from a wide variety of standard and specialized E. coli strains, including popular K12 cloning strains (GC5™, GC10™, JM109, DH5™, and NovaBlue™) and B strains (the BL21 family) used for recombinant protein expression.

The RapidTransit Transformation Kit offers two simple procedures to prepare competent cells; the Direct Addition Method and the Single-Centrifugation Method. The Direct Addition Method allows for preparation of competent cells, transformation, and recovery within a single tube or well. The Direct Addition Method provides transformation efficiencies of >1 × 10⁵ colony forming units (cfu)/μg supercoiled pUC18 plasmid DNA, whereas the Single-Centrifugation Method provides reproducible transformation efficiencies of >1 × 10⁶ cfu/μg supercoiled pUC18 plasmid DNA, when using strain JM109.

RapidTransit is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

2x RapidTransit^™ Transformation Buffer

Recovery Medium