R3131
Sigma

Bln I from Brevibacterium linens

buffered aqueous glycerol solution

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CAS Number

86352-27-4

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD01092748

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Descriptions

Caution

Enzyme in storage buffer stable at −15 to −25 °C. Store at -20 °C.

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Specificity

Recognition sequence: 5′-C/CTAGG-3′
Ligation and recutting results: After 2-10-fold Bln I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >70% of fragments can be ligated, and >90% recut.
Heat inactivation:. This enzyme is not heat inactivated at 65 °C for 15 minutes.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM KCl, 10 mM 2-mercaptoethanol, 100 μg/ml BSA, 50% glycerol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Daniels, D.L., The complete Avri l restriction map of the Escherichia coli genome and comparisons of several laboratory strains. Nucleic Acids Res. 18, 2649-2651, (1990) abstract

McClelland, M., et al., Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 15, 5985-6005, (1987) abstract

Safety

WGK Germany