R3379
Sigma

Ava I from Anabaena variabilis

buffered aqueous glycerol solution

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CAS Number

81295-06-9

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166559

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).

Linkage

Isoschizomer: BsoB I.

Specificity

Recognition sequence: 5′-C/PyCGPuG-3′
Ligation and recutting results: After 2-10-fold Ava I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-Mercaptoethanol, 0.01% Polydocanol (v/v), 50% glycerol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

5,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Murray, K., et al., Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.. Biochem. J. 159, 317-322, (1976) abstract

Safety

WGK Germany