R3635
Sigma

Bsm�I from Bacillus stearothermophilus NUB�36

buffered aqueous glycerol solution

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CAS Number

122007-72-1

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166784

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B 3657).

Comment: Optimal activity is at 65°C.

Specificity

Recognition sequences: 5'-GAATGCN/-3' 3'-CTTAC/GN 5
Ligation and recruiting results: After 2-10-fold Bsm�I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >95% recut.
Heat inactivation: Heat inactivation for Bsm I is not available.

Physical form

Solution in 10 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 1 mM dithiothreitol, 50 mM KCl, 200 μg/ml BSA, 50% glycerol (v/v) at 4 °C.

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Kessler, C., et al., Specificity of restriction endonucleases and methylases: a review. Gene 47, 1-153, (1986) abstract

Safety

WGK Germany