R4253
Sigma

BstE II from Bacillus stearothermophilus ET

buffered aqueous glycerol solution

CAS Number

93229-61-9

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166645

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SB (B8781).

Comment: BstE II is optimally active at 60°C.

Specificity

Recognition sequence: 5′-G/GTNACC-3′
Ligation and recutting results: After 2-10-fold BstE II overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: This enzyme cannot be inactivated at 65 °C for 15 minutes.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 5 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Kessler, C., et al., Specificity of restriction endonucleases and methylases--a review. Gene 47, 1-153, (1986) abstract

Safety

WGK Germany