R4258
Sigma

Apa�I from Acetobacter pasteurianus

buffered aqueous glycerol solution

CAS Number

85270-15-1

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166621

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SA (B7531).

Comment: Ad-2 DNA is the substrate for the activity, cleavage, ligation, recleavage and overdigestion assays. Optimal activity is at 30 °C.

Specificity

Recognition Sequence: 5'-GGGCC/C-3'
Ligation and recutting results: After 2-10-fold Apa�I overdigestion of Ad-2 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65�°C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-Mercaptoethanol, 0.02% Polydocanol (v/v), 50% glycerol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Seurinck, J., et al., A new restriction endonuclease from Acetobacter pasteurianus.. Nucleic Acids Res. 11, 4409-4415, (1983) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany