Ribonuclease A from bovine pancreas 

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Protein determined by E1%/280.

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.

Tested in plasmid purification. At concentrations up to 10 μg per mL, nicking or degradation of plasmid is not detectable. Detection limit: Degradation of 10% of the DNA.

Boiling of solutions to inactivate DNase is unnecessary and is not recommended.

Derived from New Zealand-sourced pancreas

Solution in 50% glycerol containing 10 mM Tris-HCl, pH 8.0

for molecular biology

buffered aqueous glycerol solution

mol wt ~13,700

suitable for plasmid purification

−20°C

Merck 13,8286

1

10-21

D5758, Diethyl pyrocarbonate