R4756
Sigma

Rsa I from Rhodopseudomonas sphaeroides

buffered aqueous glycerol solution

CAS Number

80449-06-5

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166554

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SL (B3782).

Comment: Rsa I cuts single-stranded DNA poorly.

Specificity

Recognition sequence: 5′-GT/AC-3′
Ligation and recutting results: After 2-10-fold Rsa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and 95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.

Physical form

Solution in 40 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.25% polydocanol (v/v) at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Lynn, S.P., et al., Rsa l: a new sequence-specific endonulcease activity from Rhodopseudomonas sphaeroides.. J. Bacteriol. 142, 380-383, (1980) abstract

Safety

WGK Germany

RTECS

RF0758300