R5007
Sigma

Sca I from Streptomyces caespitosus

buffered aqueous glycerol solution

CAS Number

95329-12-7

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166663

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Comment: High concentrations of Sca I may show star activity. Use minimum enzyme concentrations for complete cleavage.

Specificity

Recognition sequence: 5′-AGT/ACT-3′
Ligation and recutting results: After 2-10-fold Sca I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >90% of fragments can be ligated, and >90% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.05% polydocanol (v/v) at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Kita, K., et al., Interaction of the restriction endonuclease Scal with its substrates. Nucleic Acids Res. 13, 7015-7024, (1985) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany