Ribonuclease A from bovine pancreas 

Protein determined by E1%/280.

RNase A purity determined by SDS-PAGE

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.

A further chromatographic purification of R5503 to yield essentially pure RNAse A

Derived from New Zealand-sourced pancreas

Type XII-A

≥90% (SDS-PAGE)

lyophilized powder

mol wt ~13,700

salt, essentially free

protease, essentially free

−20°C

Merck 13,8286

3

RF0760000

D5758, Diethyl pyrocarbonate