R5509
Sigma

Nde I from Neisseria denitrificans

buffered aqueous glycerol solution

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CAS Number

84628-87-5

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166615

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Specificity

Recognition sequence: 5′-CA/TATG-3′
Ligation and recutting results: After 2-10-fold Nde I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 60 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 1 mM dithioerythritol, 0.02% polydocanol, 0.01% gelatine, 50% glycerol (v/v) at 4 °C.

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Watson, R.J., et al., Ndel: a restriction endonuclease from Neisseria denitrificans which cleaves DNA at 5 ′-CATATG-3′. FEBS Lett. 150, 114-116, (1982) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany