Acc I from Acinetobacter calcoaceticus 

Supplied with 10x Restriction Endonuclease Buffer SA (B7531).

One unit of Acc I cleaves approximately 98% of the substrate DNA (pBR322 DNA).

Recognition Sequence: 5'-GT/(A,C)(G,T)AC-3'
Ligation and recutting results: After 2-10-fold Acc I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >90% recut.
Heat inactivation: Activity retained after 10 minutes at 65 °C. Five-fold enhanced activity occurs at 55°C. Enzyme inactivated at 80 °C for 20 minutes.

Solution in 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 10 mM 2-mecarptoethanol, 0.01% polydocanol (v/v), 50% glycerol (v/v), at 4°C

buffered aqueous glycerol solution

≥5,000 units/mL

wet ice

−20°C

Roberts, R.J., Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 12, 167-204, (1984)

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

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D2886, DNA Ligase from T4-infected Escherichia coli

LIG2, QuickLink^™ DNA Ligation Kit