Monoclonal Anti-CD8−Quantum Red^™ antibody produced in mouse 

CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor's cytoplasmic portion is associated with p56^lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).

human thymocytes followed by peripheral blood T cells.

Quantum Red is a tandem fluorescent dye created by coupling R-phycoerythrin with Cy5 reactive dye. This red dye is efficiently excited at 488 nm and emits at 670 nm.

Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Quantum Red is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

purified immunoglobulin

UCHT-4

buffered aqueous solution

antigen mol wt 30/32 kDa

human

protect from light

flow cytometry: 10 μL using 1 × 10⁶ cells

IgG2a

human ... CD8A(925), CD8B(926)

wet ice

2-8°C

Fischer, A., et al., Induction of a T-cell mediated suppressor activity by soluble products from antigen-specific helper/inducer human T-cell lines. Immunology 48, 177, (1983)

Merkenschlager, M., Limiting dilution analysis of proliferative responses in human lympohocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur. J. Immunol. 18, 1653, (1988)

McMichael, A.J. Leucocyte Typing III Oxford , (1987)

3