R6501
Sigma

Ribonuclease H from Escherichia coli

recombinant, expressed in Escherichia coli, buffered aqueous glycerol solution, 1,000-4,000 units/mL

CAS Number

9050-76-4

Enzyme Commission (EC) Number

3.1.4.34 ( BRENDA | IUBMB )

MDL number

MFCD00132186

Descriptions

Unit Definition

One unit hydrolyzes 1.0 nanomole RNA in ³H-labeled poly(A) • poly(dT) to acid soluble material in 20 min at 37 °C.

Physical form

Solution in 50% glycerol containing 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 10 mM MgCl₂, 0.1 mM EDTA, 0.1 mM DTT and 0.05 mg BSA per ml

Properties

recombinant

expressed in Escherichia coli

form

buffered aqueous glycerol solution

packaging

vial of ~30 units

concentration

1,000-4,000 units/mL

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany

Related Categories

... General Metabolic Enzymes > Nucleotide Metabolism

... Application Index > Protein & Nucleic Acid Isolation

... 3.x.x.x Hydrolases > 3.1.x.x Acting on esters

MSDS

Certificate of Analysis

Certificate of Origin