R7023
Sigma

Pst I from Providencia stuartii

buffered aqueous glycerol solution

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CAS Number

81295-32-1

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00165594

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Comment: Pst I requires optimal reaction conditions in order to avoid star activity.

Specificity

Recognition sequence: 5′-CTGCA/G-3′
Ligation and recutting results: After 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Physical form

Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Malyguine, E., et al., Alterations of the specificity of restriction endonulceases in the presence of organic solvents. Gene 8, 1631, (1980)

Safety

WGK Germany