R8257
Sigma

Mlu I from Micrococcus luteus (lysodeikticus)

buffered aqueous glycerol solution

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CAS Number

81458-04-0

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166590

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Descriptions

Caution

Comment: Mlu I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam⁺ strains).

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SH (B3657).

Specificity

Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 250 mM KCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Sugisaki, H., and Kanazawa, S., New restriction endonucleases from Flavobacterium okeanokoites (Fokl) and Micrococcus luteus (Mlul). Gene 16, 73-78, (1981) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany