R8507
Sigma

Hpa I from Haemophilus parainfluenzae

buffered aqueous glycerol solution

CAS Number

81295-24-1

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00166572

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SA (B7531).

Specificity

Recognition sequence: 5′-GTT/AAC-3′
Ligation and recutting results: After 2-10-fold Hpa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >80% of fragments can be ligated, and >95% recut.
Heat inactivation: Not completely inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.5 mM EDTA, 5 mM 2-mercaptoethanol, 0.01% polydocanol, 50% glycerol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

3,000-10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Garfin, D.E., et al., Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae. Biochem. Biophys. Res. Commun. 59, 108-116, (1974) abstract

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Safety

WGK Germany