R8631
Sigma

Bcl I from Bacillus caldolyticus

buffered aqueous glycerol solution

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CAS Number

81295-11-6

Enzyme Commission (EC) Number

3.1.21.4 ( BRENDA | IUBMB )

MDL number

MFCD00163037

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Descriptions

Other Notes

Supplied with 10x Restriction Endonuclease Buffer SM (B3158).

Cleavage activity: Bcl I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam⁺ strains).
Comment: Bcl I is optimally active at 50°C.

Specificity

Recognition sequence: 5′-T/GATCA-3′
Ligation and recutting results: After 2-10-fold Bcl I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, 95% of fragments can be ligated, and 95% recut.
Heat inactivation: This enzyme cannot be heat inactivated at 65 °C for 15 minutes.

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 200 mM NaCl, 10 mM 2-Mercaptoethanol, 0.2 % Triton X-100 (v/v), 50% glycerol (v/v) at 4°C

Properties

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

References

Reference

Kessler, C., et al., Specificity of restriction endonucleases and DNA modification methyltransferases: a review (edition 3). Gene 92, 1-248, (1990) abstract

Bingham, A.H.A., et al., A specific endonuclease from Bacillus caldolyticus.. Nucleic Acids Res. 5, 3457-3467, (1978) abstract

Safety

WGK Germany