Ras human 

Package size based on protein content

A guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to MAP kinase cascade. Ras is a substrate for farnesyltransferase (FTase); when farnesylated, it is anchored to the membrane. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for new anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.

Solution in 50% glycerol containing 20 mM Tris, pH 7.6, 5 mM MgCl₂, 50 mM NaCl, 1 mM DTT

expressed in Escherichia coli

~95% (SDS-PAGE)

buffered aqueous glycerol solution

mol wt 21 kDa

≥0.25 mol/mol, H-Ras binding capacity (GDP)

dry ice

−70°C

Tucker, J., et al., Expression of p21 proteins in Escherichia coli and stereochemistry of the nucleotide-binding site. EMBO J. 5, 1351-1358, (1986) abstract

Tamanoi, F., Inhibitors of Ras farnesyltransferases. Trends Biochem. Sci. 18, 349-353, (1993) abstract

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