Thrombin CleanCleave^™ Kit 

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

• Fast, efficient cleavage in as little as 2 hours
• Thrombin is covalently bound to agarose for easy removal.
• The robust cleavage reaction is effective at temperatures from 4°C to 37 °C and over a wide range of pH and ionic strengths.
• Cleave tags even in the presence of 0.1% Triton, 1 M urea or 5 mM EDTA
• Thrombin-agarose is reusable.

200μl of a 50% slurry of thrombin-agarose cleaves >85% of 1mg of fusion protein.

CleanCleave is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

wet ice

−20°C

Di-Cera, E., et al., Linkage between proton binding and amidase activity in human alpha-thrombin: effect of ions and temperature. Biochemistry 30, 7913-7924, (1991) abstract

10× Protein Cleavage Buffer 10 mL

Disposable Spin Column 1 each