GenElute^™ Mammalian Total RNA Miniprep Kit 

The GenElute^™ Mammalian Total RNA Purification Kit combines silica-membrane technology with a convenient spin column format for a rapid bind, wash, and elute method to prepare high quality total RNA.

For additional information, please see www.sigma-aldrich.com/totalrna.

• Purifies total RNA from up to 10⁷ cells or 40 mg of tissue per prep
• Yields up to 150 μg of pure, concentrated total RNA per prep
• Recover RNA from as few as 100 cells
• Simple and efficient–12 to 18 preps in 30 minutes
• Faster than gravity flow anion exchange methods
• No cumbersome steps associated with resins and magnetic slurries
• 40% more purifications per kit than the leading supplier

Samples are lysed and homogenized in guanidine thiocyanate and 2-mercaptoethanol to release RNA and inactivate RNases. Lysates are spun through a filtration column to remove cellular debris and shear DNA. The filtrate is then applied to a high capacity silica column to bind total RNA, followed by washing and elution. Up to 150 μg of total RNA can be recovered per prep in 100 μl of water. The purified RNA is ready for Northern blots (Fig. 1), RT-PCR (Fig. 2) and other common applications.

GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

sufficient for 70 purifications

UN 3316 6.1/PG 3