SYBR^® Green JumpStart^™ Taq ReadyMix^™ for Quantitative PCR, Capillary Formulation 

• Convenient -2× concentrate ReadyMix specifically designed for use with capillary instruments such as the Roche LightCycler and is ideal for high throughput applications.
• Increased specificity & target yield - JumpStart Taq polymerase prevents non-specific product resulting in more accurate C_T values and improved quantitation.

SYBR Green JumpStart Taq ReadyMix, Capillary formulation combines the advantages of a hot start enzyme, JumpStart taq, in a 2× concentrate ReadyMix specifically designed for use with capillary instruments, such as the Roche LightCycler® real-time thermal cycler. SYBR Green JumpStart Taq ReadyMix is an optimized formulation containing SYBR Green I dye, JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer and stabilizers.
SYBR Green Taq ReadyMix is recommended for single product real-time amplification experiments and may also be used for evaluation of primer sequences prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

A tube of 25 mM MgCl₂ is provided for easy optimization of the QPCR reaction.

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Specificity of Sigma's SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.
The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate C_T values.
To prepare a reaction, 10 μl of ReadyMix is added to primers, template and water for a final reaction volume of 20 μl.

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR^® Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR^® is a registered trademark of Molecular Probes, Inc.

ReadyMix is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

SYBR is a registered trademark of Molecular Probes, Inc.

1 units/reaction (20 μL reaction volume)

wet ice

−20°C

Z702439, A-Z of Quantitative PCR