Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse 

bovine brain S-100b

Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca²⁺-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.

ascites fluid

SH-B4

15 mM sodium azide

human, bovine, pig, rabbit, goat, sheep, canine, feline, rat

dot blot: suitable using denatured-reduced preparations

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using protease-digested, formalin-fixed, paraffin-embedded human tongue sections

indirect ELISA: suitable

IgG1

rat ... S100b(25742)

human ... S100B(6285)

dry ice

−20°C

3