Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse 

partially purified mammalian splicesome.

Recognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification.

ascites fluid

SC-35

antigen mol wt 35 kDa (a doublet)

15 mM sodium azide

human, rat, newt, frog, Drosophila

immunocytochemistry: suitable

immunoprecipitation: suitable

indirect ELISA: suitable

indirect immunofluorescence: 1:2,000 using cultured human fibroblasts

IgG1

human ... SFRS2(6427)

rat ... Sfrs2(494445)

dry ice

−20°C

Fu, X.-D., and Maniatis, T.I., Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437-441, (1990) abstract

Fu, X.-D., et al., General splicing factors SF2 and SC35 have equivalent activities in vitro and both affect alternative 5' and 3' splice site selection. Proc. Natl. Acad. Sci. USA 89, 11224, (1992) abstract

Spector, D.L., et al., Associations between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 10, 3467-3481, (1991) abstract

Fu, X.-D., and Manatis, T.I. Science 256, 535-538, (1992) abstract

Wang, J., et al., Localization of pre-messenger RNA at discrete nuclear sites. Proc. Natl. Acad. Sci. USA 88, 7391-7395, (1991) abstract

Carmo-Fonseca, M., et al., Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies. J. Cell Biol. 117, 1-14, (1992) abstract

Gui, J.-F., et al., A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature 369, 678-682, (1994) abstract

Fu, X.-D., and Maniatis, T.I., The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site. Proc. Natl. Acad. Sci. USA 89, 1725-1729, (1992) abstract

Jimenez-Garcia, L.F., and Spector, D.L., In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73, 47-59, (1993) abstract

Wu, Z., et al., Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes. J. Cell Biol. 113, 465-483, (1991) abstract

Carmo-Fonseca, M., et al., In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells. EMBO J. 10, 1863-1873, (1991) abstract

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