β-Secretase human 

One unit will hydrolyze 1.0 picomole of 7-methoxycoumarin-4-acetyl-[Asn⁶⁷⁰, Leu⁶⁷¹]-Amyloid β/A4 Precursor Protein 770 Fragment 667-676-(2,4-dinitrophenyl)-Lys-Arg-Arg amide substrate per 1 minute at pH 4.5 at 37 °C.

Solution in 20 mM Hepes, pH 7.4, 125 mM NaCl.

BACE1 (β−Secretase or β−Site APP-Cleaving Enzyme) is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer's, therefore BACE1 is a target for inhibitor drug discovery.

expressed in HEK 293 cells (C-terminal FLAG-tagged)

≥90% (SDS-PAGE)

buffered aqueous solution

human ... BACE1(23621)

wet ice

2-8°C

Selkoe, D.J., Cell biology of protein misfolding: the examples of Alzheimer's and Parkinson's diseases. Nat. Cell Biol. 6, 1054-1061, (2004) abstract

-