SigmaSpin^™ Sequencing Reaction Clean-Up 

Ideal for removing dye-terminator nucleotides and primers from sequencing reactions and radiolabeled nucleotides, primers, and fluorescent dyes from nucleic acid probe labeling reactions.

• Validated with many common automated sequencers and dye terminator chemistries
• Pre-qualified size-exclusion resin guarantees optimum performance
• Unique drip directors prevent cross-contamination between samples during collection
• Plates are sealed to eliminate leakage or drying during shipping or storage
• Suitable for use with multi-channel pipettes and automated workstations

SigmaSpin Post-Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean-up of probe labeling reactions or small numbers of sequencing reactions. These columns can accept sample volumes up to 100 μl. Each column comes with a collection tube to collect the DNA during centrifugation.

SigmaSpin 96-Well Post-Reaction Clean-Up plates provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components. Each plate is packed with a pre-hydrated size-exclusion resin, equilibrated with molecular biology grade water, and supplied in our unique plate design with long drip directors to minimize contamination between samples. The plate design also includes a snap-cap bottom seal and a foil seal top to ensure that the resin remains hydrated. SigmaSpin has been tested in high-throughput genome centers and core facilities with ABI Prism^® 3700, 3100, 310 and 377. Each well can accept sample volumes up to 20 μl.

For additional information, please see www.sigma-aldrich.com/sequencing.

SigmaSpin is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

50 Post-Reaction Clean-Up Plates

2-8°C

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